Refine your search
Collections
Co-Authors
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Chattaraj, Shruti
- Isolation of Mesophyll Protoplast from Indian Mulberry (Morus alba L) CV. S1635
Abstract Views :352 |
PDF Views:0
Authors
Affiliations
1 Division of Plant Biology, Bose Institute, P-1/12, C.I.T., Scheme VII M, Kolkata-700 054, West Bengal, IN
1 Division of Plant Biology, Bose Institute, P-1/12, C.I.T., Scheme VII M, Kolkata-700 054, West Bengal, IN
Source
Journal of Environment and Sociobiology, Vol 13, No 2 (2016), Pagination: 217-222Abstract
Mulberry (Morus alba L. cv S1635) is a perennial crop plant in Indian sericulture and used for silkworm Bombyx mori L. feeding. Qualitative and quantitative improvement of this crop is highly essential in respect to better and quality silk production and hence through breeding approaches the development of new lines is important. Protoplasts are the good source of any plant to study about their genetics and cellular mechanisms for breeding. Using cell wall degradating enzymes mulberry protoplasts from mesophyll tissue were isolated. Several combinations of cellulose, macerozyme and driselase enzymes mixture were tested in different incubation conditions. A combination of Cellulase R10-1%, Macerozyme R10-1% and Driselase-0.25% in 0.6M mannitol showed comparatively better yield (3.8 × 106 / gm tissue) after 3-4 hrs shaking with 100 rpm at 28°C.Keywords
Mulberry Plant, Mesophyll Protoplast, Cell Wall Degradating Enzymes.
References
- Asai, T., Stone, J. M., Heard, J. E., Kovtun, Y., Yorgey, P., Sheen, J. and Ausubel, F. M. 2000. Fumonisin B1-induced cell death in Arabidopsis protoplasts requires jasmonate-, ethylene-, and salicylate-dependent signalling pathways. Plant Cell, 12: 1823-1835.
- Bethke, P. C. and Jones, R. L. 2001. Cell death of barley aleurone protoplasts is mediated by reactive oxygen species. Plant J., 25: 19-29.
- Castro, A. 1989. Producción de leche de cabrasalimentadas con King grass (Pennisetum purpureum x P. typloides), suplementadas con diferentesniveles de follaje de Poró (E. poeppigrama) y de fruto de plátano (Musa sp. var. pelipita). Ph.D. thesis, University of Costa Rica, CATIE, 58 pp.
- Cocking, E. C. 1960. A method for the isolation of plant protoplasts and vacuoles. Nature, 187: 927-929.
- Cocking, E. C. 2000.Turning point article plant protoplasts, in vitro cell. Dev. Biol. Plant, 36: 77-82.
- Davey, M. R., Anthoney, P., Brian Power, J. and Love, K. C. 2005, Plant protoplants: status and biochemical perspectives. Biotechnology Advances, 23: 131–171.
- Katagiri, K. 1988. Differences in cell division in mulberry mesophyll protoplast culture due to species and culture conditions. J. Seri. Sci. Japan, 57: 445–446.
- Katagiri, K. 1989. Colony formation in culture of mulberry mesophyll protoplasts. J. Seric. Sci. Japan, 58: 267–268.
- Kim, B. K., Kang, J. H., Jin, M., Kim, H. W., Shim, M. J. and Choi, E. C. 2000. Mycelial protoplast isolation and regeneration of Lentinus lepideus. Life Sci., 66: 1359-1367.
- Koster, K. L., Reisdorph, N. and Ramsay, J. L. 2003. Changing desiccation tolerance of pea embryo protoplasts during germination. J. Exp. Bot., 54: 1607-1614.
- Koyuncu, F. 2004. Organic acid composition of black mulberry. Chemistry of Natural Compounds, 40: 368-369.
- Mandal, P. and Sikdar, S. R. 2003. Plant regeneration from mesophyll protoplasts of Rorippa indica (L.) Hiern, a wild crucifer. Current Science, 85: 1451-1454.
- Ohnishi, T. and Kiyama, S. 1987. Increase in yield of mulberry protoplasts by treatment with chemical substances. J. Seric. Sci. Japan, 56: 407–410.
- Ohyama, K. and Oko, S. 1975. Isolation of protoplasts in Moraceae. Proc. Crop. Sci. Soc. Japan, 44: 121–122.
- Sheen, J. 1999. C4 gene expression. Ann. Rev. Plant Physiol. Plant Mol. Biol., 50: 187-217.
- Sheen, J. 2001. Signal transduction in maize and Arabidopsis mesophyll protoplasts. Plant Physiol., 127: 1466-1475.
- Tena, G., Asai, T., Chiu, W. L. and Sheen, J. 2001. Plant MAP kinase signaling cascades. Curr. Opin. Plant Biol., 4: 392-400.
- Tewary, P. K. and Lakshmisita, G. 1992. Protoplast isolation, purification and culture in mulberry (Morus spp.). Sericologia, 32: 651–657.
- Tewary, P. K., MohanRam, H. Y. and Rangaswamy, N. S. 1995. Isolation and culture of mulberry (Morus alba L.) protoplasts from callus tissues. Ind. J. Plant Physiol., 38: 114–117.
- Tohjima, F., Yamanouchi, H., Koyama, A. and Machii, H. 1996. Effects of various enzymes on the efficiency of protoplast isolation from mulberry mesophylls. J. Seric. Sci. Japan, 65: 485–489.
- Umate, P., Rao, K. V., Krianmayee, K., Jaya Sree, T. and Sadanandam, A. 2005. Plant regeneration of mulberry (Morus indica) from mesophyll-derived protoplasts. Plant Cell Tissue Organ Cult., 82(3): 289–293.
- Wei, Z., Xu, Z., Huang, J., Xu, N. and Huang, M. 1994. Plants regenerated from mesophyll protoplasts of white mulberry. Cell Research, 4: 183–189.
- Yanagisawa, S., Yoo, S. D. and Sheen, J. 2003. Differential regulation of EIN3 stability by glucose and ethylene signalling in plants. Nature, 425: 521–525.
- Widholm, J. M. 1972. The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Tech., 47: 189–194.
- Fatty Acid Profile Analysis of Abci Somatic Hybrid Mushroom Strains using GC-MS Technique
Abstract Views :240 |
PDF Views:1
Authors
Affiliations
1 Division of Plant Biology, Bose Institute, P-1/12, C.I.T. Scheme VII M, Kolkata-700 054, IN
1 Division of Plant Biology, Bose Institute, P-1/12, C.I.T. Scheme VII M, Kolkata-700 054, IN